Figure 4.

ZFP91 promotes non‐canonical caspase‐8 inflammasome complex assembly in LPS‐stimulated THP‐1 cells. (A) PMA‐differentiated THP‐1 cells were transduced with lentiviruses containing ZFP91 gene (MOI = 100) for 24 h and then stimulated with 1 μg·mL⁻¹ LPS for 6 h. Immunoblot analysis of NLRP3, caspase‐8, ZFP91 and ASC after immunoprecipitation of proteins with antibody to ASC. (B) PMA‐differentiated THP‐1 cells were transduced with lentiviruses carrying siRNA against ZFP91 (MOI = 100) for 24 h and then stimulated with 1 μg·mL⁻¹ LPS for 6 h. Immunoblot analysis of NLRP3, caspase‐8, ZFP91 and ASC after IP of proteins with antibody to ASC. (C) PMA‐differentiated THP‐1 cells were transduced with lentiviruses containing ZFP91 gene (MOI = 100) for 24 h and then stimulated with 1 μg·mL⁻¹ LPS for 6 h. Immunofluorescence colocalization was analysed by immunofluorescence cytochemistry. Nuclei were stained with DAPI. Original magnification: ×600.