Figure 1.

Effect of treatment with AM6545 and/or perindopril on CB₁, CB₂ and AT₁ receptors in DM. Control ND and DM were treated with vehicle, AM6545 (AM), perindopril (ACE‐I) or AM6545 plus perindopril (Combo) (n = 6 mice per group). Representative immunohistochemistry analysis of CB₁ receptors (A) and CB₂ receptors (D) are shown (original magnification ×400) and quantification of glomerular positive staining reported in the graphs (B–E). CB₁, CB₂ and AT₁ receptor mRNA levels were measured by real‐time PCR on total RNA extracted from the renal cortex. Results were corrected for the expression of the housekeeping gene HPRT and shown in the graphs (C, F, I). Representative immunoblot (G) and densitometry analysis (H) of AT₁ receptor protein expression in total protein extracts from the total renal cortex are shown. Tubulin was used as internal control (*P < 0.05, vs. ND).