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. 2018 Nov 6;175(23):4371–4385. doi: 10.1111/bph.14495

Figure 4.

Figure 4

Effect of AM6545 and/or perindopril on diabetes‐induced inflammation. ND and DM were treated with vehicle, AM6545 (AM), perindopril (ACE‐I) or AM6545 plus perindopril (Combo) (n = 6 mice per group) for 14 weeks. Representative images of glomerular F4/80 (A) and MAC‐2 (C) positive immunostaining are shown and quantitation reported in the graph (B, D). Total RNA was extracted from the renal cortex and levels of mRNA encoding for CD68 (E), ICAM‐1 (F), MCP‐1 (H) and CCR2 (J), measured by real‐time PCR. Protein levels of ICAM‐1 (G) and MCP‐1 (I) in renal tissue were determined by elisa and normalized to total protein (*P < 0.05 vs. ND; # P < 0.05 vs. DM group; °P < 0.05 dual vs. single treatment; § P < 0.05 combo vs. ND).