Table 2.
Saccharomyces cerevisiae strains used in this study.
| Strain | Relevant genotype | Description | Reference |
|---|---|---|---|
| CEN.PK113-7D | MATa | Reference strain | (Entian and Kötter 2007) |
| CEN.PK113-5D | MATa ura3-52 | Uracil auxotrophic reference strain | (Entian and Kötter 2007) |
| CEN.PK102-3A | MATA ura3-52 leu2-112 | Uracil and leucine auxotrophic strain | (van Dijken et al.2000) |
| RWB217 | CEN.PK102-3A loxP-pTPI::(−266, −1)TAL1 gre3Δ::hphMX pUGpTPI-TKL1 pUGpTPI–RPE1 KanloxP–pTPI::(−40, −1)RKI1 {pAKX002, p415ADHXKS} | Metabolically engineered, non-evolved xylose consuming strain | (Kuyper et al.2005) |
| IMX975 | RWB217 can1::CAS9-tagA-loxP-natNT2-loxP | RWB217 expressing Cas9 | This study |
| IMX1366 | IMX975, sga1::pPGI1-NQM-TagB-pPYK1-TKL2 | IMX975 over-expressing NQM1 and TKL2 | This study |
| IMX581 | CEN.PK113–5D can1::CAS9-tagA-loxP-natNT2-loxP | CEN.PK113–5D expressing S. pyogenes Cas9 | (Mans et al.2015) |
| IMX696 | IMX581 gre3::pTDH3-RPE1- pPGK1-TKL1 – pTEF1-TAL1- pPGI1-NQM1 – pTPI1-RKI1 –pPYK1-TKL2-(pTPI1-xylA-tCYC1)*36)-pTEF-XKS1, pUDE335 | IMX581 over-expressing PPP genes incl. NQM1 and TKL2, expression of Piromyces xylA based on 36 integrated copies | (Verhoeven et al.2017) |
| IMX994 | IMX581 gre3::pTDH3-RPE1-tagH-pPGK1-TKL1-TagI-pTEF1-TAL1-TagA- pTPI1-RKI1-TagL- pTEF-XKS1 | IMX581 over-expressing genes from the non-oxidative pentose phosphate pathway | (Papapetridis et al.2018) |
| IMU079 | IMX994, pAKX002 (2 μm xylA) | IMX994 over-expressing xylA from a 2 μm plasmid | (Papapetridis et al.2018) |
| IMX1456 | IMX994, sga1::pPGI1-NQM-TagB-pPYK1-TKL2 | IMX994 over-expressing NQM1 and TKL2 | This study |
| IMU081 | IMX1456, pAKX002 (2 μm xylA) | IMX1456 over-expressing xylA from a 2 μm plasmid | This study |
| IMX800 | IMX581, gre3::pTDH3-RPE1-TagH-pPGK1-TKL1-TagI-pTEF1-TAL1-TagA-pPGI1-NQM1-TagB- pTPI1-RKI1-TagC-pPYK1-TKL2-TagL- pTEF-XKS1 | IMX581 over-expressing PPP genes incl. NQM1 and TKL2 | This study |
| IMU078 | IMX800, pAKX002 (2 μm xylA) | IMX800 over-expressing xylA from a 2 μm plasmid | This study |
| IMX1736 | IMU078, pck1Δ | IMU078 with a deletion in PCK1 | This study |