Fig. 1.

Schematic illustration of luciferase-linked antibody capture assay (LACA); a Black 96-well polystyrene microplates were coated with 1 μg of recombinant Protein G; b Microplate coated with protein G was blocked by PBS-T buffer containing 2.5% gelatin; c The 20-fold diluted serum samples and PBS-diluted HEK293T cell lysates containing 1 TU Renilla luciferase-fused antigen were mixed together and incubated in Protein G-coated well for 2 h at room temperature (RT); d Renilla luciferase activity was determined by adding substrate to each well followed by measurement of luciferase activity using a VICTORX™ X5 Multilabel Reader