Fig. 2.

Down-regulation of BKCa expression partially attenuated increased phosphorylation of MEK1/2 and ERK1/2 induced by E2, and reversed the alterations to E2 mediated biological effects in Ishikawa. a Down-regulated expression of BKCa using siRNA partially attenuated the increase of p-MEK1/2 (p < 0.01) and p-ERK1/2 (p < 0.01) expression induced by E2 stimulation. b Down-regulated expression of BKCa inhibited the proliferation of Ishikawa cell, whereas 1 nM E2 treatment accelerated the cells growth. Decreased expression of BKca partially attenuated the increased proliferative effects induced by E2 (p < 0.05). c The reduction in apoptosis by E2 was reversed by siRNA targeting BKCa (p < 0.01). d Down-regulation of BKCa attenuated the altered cell cycle distribution induced by E2 (p < 0.01). e, f The increased migration and invasion of Ishikawa stimulated by E2 were both reversed by knock down of BKCa expression (p < 0.01). Data (Mean ± SD, n = 3 independent experiments)