Fig. 5.

Effects of a forced reversal of the miR-199a-3p or AK4 levels on the esophageal cancer cells. The cells were transfected for 24 h, then cells were digested and counted according to 0 Gy (500), 2 Gy (1000), 4 Gy (2000), 6 Gy (5000), 8 Gy (8000) cells/well and was inoculated in a 6-well plate in triplicate, the corresponding dose was irradiated after 24 h, using a 6-MV x-ray generated by a linear accelerator Varian trilogy at a dose rate of 2 Gy/min (varian trilogy at a dose rate of 2 Gy/min). a, b MiR-199a-3p mimic (3PM)-transfected Kyse30 and Kyse150 increases survival fraction versus the negative control (NC) cells. c, d MiR-199a-3p antagomiR (3PA)-transfected Kyse30-R and Kyse150-R decreases NC cells survival fraction versus the negative control (NC) cells. AK4 protein level (western blot analysis) and mRNA determined by qRT-PCR in the si-AK4-transfected versus the NC-transfected Kyse30 and Kyse150 cells e, f. Si-AK4-transfected Kyse30 and Kyse150 cells increases NC cells survival fraction versus the negative control (NC) cells g, h. The surviving fraction was calculated using the multitarget single-hit model: Y = 1 − (1 − exp(− k * x))^N. The data are presented as the mean ± standard deviation of results from 3 independent experiments, and two way anova was used to calculate statistical significance