Fig. 6.

The effects of the forced reversal of miR-199a-3p levels on the activity of the signaling pathways on the esophageal cancer cells. a Relative activities (mean ± SD) of the seventeen pathways in Kyse30, Kyse150 Kyse30-R and Kyse150-R cell lines. Three pathways of TGFβ, MAPK/JNK and IL-6 with consistent change trend between Kyse30/Kyse30-R and Kyse150/Kyse150-R. b The relative pathway activities of the three pathways in the AK4 siRNA versus the NC-transfected Scaber and Kyse30, Kyse150 cells. The relative expression ratio of the four transcription factors in the AK4 siRNA versus the NC-transfected Scaber and Kyse30, Kyse150 cells by qRT-PCR analyses were shown in Table (c) and in plot (d) (NC was normalized)