Table 3.

Expression analysis^a of selected Clonostachys rosea S8A serine protease genes during interaction with fungal prey

	prs1	prs2	prs3	prs4	prs5	prs6	prs7	prs8	prs9	prs10	prs11	prs12	rs13	prs14	prs15	prs16	prs17	prs18
Cr-Fg	0.57 a	0.43 b	0.49 b	0.52 a	N/D	1.47 a	1.74 a	N/D	0.63 ab	N/D	0.53 b	N/D	0.48 b	0.53 b	1.54 a	0.84 a	N/D	1.48 a
Cr-BC	1.01 a	0.35 b	0.74 ab	0.77 a	N/D	1.26 ab	1.04 a	D	0.33 b	N/D	0.56 b	N/D	0.94 ab	0.24 b	0.83 b	0.71 a	1.29 a	0.98 a
Cr-Cr	1.27 a	1.08 a	1.03 a	1.13 a	N/D	1.01 b	1.24 a	N/D	1.15 a	D	1.02 a	N/D	1.10 a	1.23 a	1.12 ab	1.04 a	8.26 a	1.11 a

N/D no detectable expression, D detectable expression

^aGene expression of S8A serine protease genes was determined by RT-qPCR during the interaction of C. rosea with B. cinerea (Cr-Bc), C. rosea with F. graminearum (Cr-Fg) and C. rosea with itself (Cr-Cr, control). Relative expression is calculated as the ratio between the target gene and tubulin using the 2^–ΔΔCT method. Different letters indicate significant differences (P ≤ 0.05) between treatments for each gene as determined by the Fisher’s least significant difference (LSD) test. The statistical analysis was performed on a minimum of three biological replicates