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. 2018 Nov 16;10:143. doi: 10.1186/s13148-018-0576-y

Table 1.

Sequences of primers employed for real-time qPCR assay

Gene Forward primer sequence Forward primer sequence Start End
Respect TSS (bp)
Vcl 5′-ATGCCAGTGTTTCATACGCG-3′ 5′-CGCCCTCCTCGTGCATTAT-3′ + 94 + 184
Gapdh 5′-TTCGCTCTCTGCTCCTCCTG-3′ 5′-CCTAGCCTCCCGGGTTTCTC-3′ + 95 + 185
Hapln1 5′-TCGGATGCTCTCAAGTTCTGC-3′ 5′-TCGCCCAGAGACAAACTTAAGG-3′ + 177 + 267
Col2a1 5′-CTTTCGAGGCTGGCGAACT-3′ 5′-CGGTTCAGGTTACAGCCCA -3′ − 86 − 16