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. 2018 Nov 17;17:159. doi: 10.1186/s12943-018-0907-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Tob1 promotes colon cancer cell proliferation via a Wnt/β-catenin signalling feedback loop. a Tob1 expression analysis by qRT-PCR and WB. b-c Effects of Tob1 knockdown or overexpression on cell proliferation analysed by MTT. d Wnt activity analysis by the TOPFlash/FOPFlash assay. e mRNA expression analysis of Wnt/β-catenin target genes by qRT-PCR. f β-Catenin levels in cells treated with CHX (10 μg/ml). g Subcellular localization analysis of Tob1 by nucleocytoplasmic separation. h Interaction analysis of β-catenin and Tob1 by co-IP. i β-Catenin expression analysis by WB. j β-Catenin regulated the expression but not phosphorylation of Tob1. k Tob1 expression was upregulated by LiCl in RKO cells. *, P < 0.05; **, P < 0.01 and ***, P < 0.001 vs control