Fig. 2.

RAB25 expression reduced ZEB2-driven cell migration. a–d ZEB2 was induced in MCF7 and A431 (+dox), and RAB25 expression was rescued by a transiently transfected RAB25 expression construct (+dox/RAB25). a, c mRNA expression level of ZEB2 and RAB25 was analyzed by qRT-PCR in (a) MCF7 and c A431. Control values were set to 1, and s.d. is shown. b, d Cell migration was evaluated by a transwell assay with b MCF7 and d A431. Results are expressed as the total number of cells counted per chamber. e–f ZEB2 was knocked down in MDA-MB-231 (shZEB2/pLVTH), and RAB25 induction was blocked using specific shRNA (shZEB2/shRAB25). e mRNA expression level of ZEB2 and RAB25 was analyzed by qRT-PCR. Control values were set to 1, and s.d. is shown. f Cell migration was evaluated by a transwell assay. Results are expressed as the total number of cells counted per chamber. For all analyses, p values were determined using one-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001). Values represent the means of three independent experiments