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. 2018 Mar 29;218(5):698–706. doi: 10.1093/infdis/jiy175

Figure 1.

Figure 1.

Mean strand-transfer activities (±standard errors of the mean) of purified recombinant wild-type integrase (WT) and integrase bearing the S230R substitution (S230R). *P < .05 compared to WT, by the Student t test (OpenEpi Toolkit). A, Strand-transfer activity in the presence of 20 nM target DNA and increasing concentrations of purified integrases. For each protein concentration, assays were performed twice independently, in triplicate, yielding 6 independent values. B, Strand-transfer activity in the presence of 400 nM purified WT or S230R integrase and increasing concentrations of target DNA. For each protein, assays were performed 3 times independently, in triplicates, yielding 9 independent values for each target DNA concentration and protein. C, Calculated maximum strand-transfer activities (Vmax) for WT and S23OR integrases, using protein concentrations specified in panel B. D, Half of the concentration of DNA necessary to reach Vmax (Km) for purified WT and S23OR integrases, using values from panel B.