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. 2018 Mar 29;218(5):698–706. doi: 10.1093/infdis/jiy175

Table 1.

Comparison of Strand-Transfer Activity of Purified Recombinant Wild-Type and S230R Integrase

Recombinant Enzyme V max, RFU/h, Mean (95% CI) P a K m, nM, Mean (95% CI) Fold Change P a V max/Km Fold Change
Wild type 25917 (24341–27494) 8.9 (6.97–10.80) 1 2918 1
S230R 21287 (19572–23001) .001 19.9 (15.26–24.55) 2.26 .001 1070 0.37

Each experiment was repeated 3 times in triplicate for each integrase concentration.

Abbreviations: CI, confidence interval; Km, half of the concentration of DNA necessary to reach Vmax; RFU, relative fluorescence units; Vmax, maximal strand-transfer activity.

aCompared to wild-type enzyme, by the Student t test (OpenEpi Toolkit). Values < .05 are considered statistically significant.