Table 2.

In Vitro Susceptibilities of Purified Recombinant Integrase to Dolutegravir (DTG), Cabotegravir (CAB), Raltegravir (RAL), and Elvitegravir (EVG) in Biochemical Assays

Recombinant Enzyme	DTG			CAB			RAL			EVG
	K i, nM, Mean ± SEM	Relative Fold Change	P a	K i, nM, Mean ± SEM	Relative Fold Change	P a	K i, nM, Mean ± SEM	Relative Fold Change	P a	K i, nM, Mean ± SEM	Relative Fold Change	P a
Wild type	1.27 ± 0.13	1		1.76 ± 0.41	1		6.40 ± 0.5	1		2.30 ± 0.13	1
S230R	3.33 ± 0.22	2.62	.004	3.38 ± 0.55	1.92	.08	13.8 ± 5.5	2.16	.3	7.34 ± 0.47	3.19	.009

Each experiment was repeated 3 times in triplicate for each drug concentration.

Abbreviations: K_i, inhibitor constant; SEM, standard error of the mean.

^aCompared to wild-type enzyme, by the Student t test (OpenEpi Toolkit). Values < .05 are considered statistically significant.