Figure 3. Hes4 expression decreases osteoblastic differentiation.
Alizarin Red S staining of HOS-GFP and HOS-GFP-Hes4 cells was performed after 21 days in differentiation media. (A) Representative images of Alizarin Red S staining within 24 well plates. (B) Mean number of foci of calcium deposition per 24-well plate well (± SEM; n = 3). *P < 0.05.