Figure 5.

The CAMK2G‐NLS^Arg292Pro mutation interferes with nuclear translocation of CAMK2G‐NLS. (A and C) Representative confocal images of hippocampal (A) or cortical (C) neurons transfected on DIV7 with CAMK2G‐NLS^WT, CAMK2G‐NLS^Arg292Pro (R292P), CAMK2G‐NLS^Ser334Ala (S224A), or CAMK2G‐NLS^{Arg292Pro/Ser334Ala} (R292P/S334A). Transfected neurons are identified by the tdTOMATO (red). (B and D) Summary bar graphs of CAMK2G expression level measured as a ratio nucleus vs. cytoplasm and normalized to the WT level. (B: CAMK2G^WT vs. CAMK2G^Arg292Pro, P = 6.81E‐19, t(75) = 11.87, two‐tailed unpaired t‐test; D: one‐way ANOVA, F[3,86] = 43.58, P = 3.19E‐17; CAMK2G‐NLS^WT vs. CAMK2G‐NLS^Arg292Pro, P = 0.0001; CAMK2G‐NLS^Ser334Ala, P = 0.99; CAMK2G‐NLS^{Arg292Pro/Ser334Ala}, P = 0.0001; CAMK2G^Arg292Pro vs. CAMK2G‐NLS^Ser334Ala, P = 0.0001; CAMK2G‐NLS^{Arg292Pro/Ser334Ala}, P = 0.99; CAMK2G‐NLS^Ser334Ala vs. CAMK2G‐NLS^{Arg292Pro/Ser334Ala}, P = 0.0001). Data are presented as mean ± SEM. Numbers (X/Y) depicted in the bar graphs represent the total number of cells (X) and number of independent cultures (Y) analyzed