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Concentration of Activin A in PBS containing unpolymerized acrylated-Activin A solution (Acryl-Activin A) and in PBS containing hydrogels with Activin covalently bound to the surface of high and low modulus hydrogels (HIGHcov and LOWcov, respectively). There was very little Activin A in the PBS and based on this concentration, it was determined that 220 ng and 206 ng of Activin was conjugated to the high and low modulus scaffold surface, respectively.
