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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Biomol Struct Dyn. 2018 May 17;37(7):1685–1699. doi: 10.1080/07391102.2018.1464957

Figure 5. (A). Antagonistic effects of SR-49059 and Manning compound on cV1aR and cV1bR mRNA expression.

Figure 5.

Dispersed chicken primary AP cells (0.5 × 106) were pretreated with SR-49059 (100 pM) or the Manning compound (100 pM) for 30 min followed by AVT/CRH (1.0 / 0.1 nM) treatments. Six hours later, total RNAs were extracted and real time RT-PCR was performed for cV1aR and cV1bR mRNA expression. Data were set as the fold changes of relative expression levels using the ΔΔCt method with GAPDH and β-actin as internal controls. Different lower-case letters above the bars denote significant differences (P<0.05) among groups. (B). Antagonistic effects of SR-49059 and Manning compound on cCRH-R1 and cCRH-R2 mRNA expression. Dispersed chicken primary AP cells (0.5 × 106) were pretreated with SR-49059 (100 pM) or Manning (100 pM) for 30 min followed by AVT/CRH (1.0 / 0.1 nM) treatments. Six hours later, total RNAs were extracted and real time RT-PCR was performed for cCRH-R1 and cCRH-R2 mRNA expression. Data were set as the fold changes of relative expression levels using the ΔΔCt method with GAPDH and β-actin as internal controls. Different lowercase letters above the bars denote significant differences (P<0.05) among groups.