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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 May 17;143(2):669–680.e12. doi: 10.1016/j.jaci.2018.05.003

Figure 8. R321 binds CCR3 in plasma membrane in the presence of CCL11.

Figure 8

13C HSQC spectra of 13C-reductively methylated CCR3 positive and CCR3 null membranes were recorded with/without 1 μM CCL11 in the presence/absence of 2 μM R321. Spectral comparisons between (A) CCR3 (CCR3-K-di13CH3)(red) and CCR3 null membranes (blue); (B) CCR3 alone (CCR3-K-di13CH3) (red) and CCR3 + CCL11 (blue); (C) CCR3 alone (CCR3-K-di13CH3) (red) and CCR3 + R321 (blue); (D) CCR3 alone (CCR3-K-di13CH3) (red) and CCR3 + CCL11 and R321 (blue); (E) CCR3 + CCL11 (red) and CCR3 + CCL11 and R321 (blue); and (F) CCR3 + R321 (red) and CCR3 + CCL11 and R321 (blue) show line-broadening and chemical shift changes indicative of binding. Black arrows show significant changes in CCR3-associated signals, but not in the signals that belong to other membrane proteins.