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. Author manuscript; available in PMC: 2018 Nov 18.
Published in final edited form as: J Bone Miner Res. 2018 Jan 26;33(4):691–703. doi: 10.1002/jbmr.3342

Figure 4. Conditional knockout of C/ebpα significantly reduced the expression of OC regulator genes (ie. Nfatc1, c-fos) and OC function genes (ie. Ctsk, Atp6i).

Figure 4.

(A–D) Immunostaining using antibodies against (A) C/ebpα, (B) Ctsk, (C) c-Fos, (D) PU.1 and (E) Nfatc1 and their corresponding TRAP+ co-stained images for newborn C/ebpαf/fLysM-Cre (f/f/Δ) and C/ebpαf/f (f/f) mouse femurs. IHC analyses were also carried out using LysM-Cre mice showing similar results to C/ebpαf/f group. Lower panels in A-D show high magnification images of immunostaining results. (F) Quantification data for TRAP+ cells. (G) Quantification data of staining results in A-E. Number of positive cells were quantified per mm2 plate area using NIH image software (ImageJ). Bars show means ± SD with n=30. *p<0.05, **p<0.01.