Table 1, Key Table.

BphP derived NIR FPs with demonstrated in vivo and live-cell applications and far-red GFP-like FPs.

NIR FP	Ex, nm	Em, nm	Extinction coefficient, M−1cm−1	Quantum yield, %	Molecular brightness a	Molecular brightness vs. iRFP713, %	Oligomeric state	Photostability in mammalian cells, t1/2, s	pKa	Brightness in HeLa cells vs. iRFP713, % b	Reference
miRFP670	642	670	87,400	14.0	12.2	198	monomer	490 (155)	4.5	72	[18]
miRFP703	674	703	90,900	8.6	7.8	127		650 (394)	4.5	37
miRFP709	683	709	78,400	5.4	4.2	69		500 (192)	4.5	30
mIFP bc	683 (683)	705 (704)	65,900 (82,000)	6.9 (8.4)	4.6	74		90 (54)	4.5	15	[22]
IFP2.0 bc	688 (690)	709 (711)	72,900 (98,000)	6.8 (8.1)	5.0	80	dimer d	150 (108)	4.5	8	[21, 22]
iRFP670	643	670	114,000	12.2	13.9	225	dimer	290	4.5	119	[17, 21]
iRFP682	663	682	90,000	11.1	10.0	162		490	4.5	105
iRFP702	673	702	93,000	8.2	7.6	124		630	4.5	61
iRFP713 (aka iRFP)	690	713	98,000	6.3	6.2	100		960	4.5	100	[16]
iRFP720	702	720	96,000	6.0	5.8	93		490	4.5	112	[16, 17]
miRFP670–2	643	670	103,000	13.6	14.0	227	monomer	310	4.5	72	[29]
miRFP682	663	682	91,000	11.2	10.2	165		500	4.5	117
miRFP702	673	702	88,000	8.1	7.1	115		640	4.5	37
miRFP713	690	713	99,000	7.0	6.9	112		980	4.5	109
miRFP720	702	720	98,000	6.1	6.0	97		510	4.5	116	[19]
mCherrye	587	610	72,000	22.0	15.8	255	monomer	n.a.	3.8	n.a.	[19, 28]
FusionRede	580	608	83,000	19.0	15.8	255		n.a.	4.6	n.a.	[28, 37]
mNeptunee	600	650	57,500	20.0	11.5	185	dimer f	n.a.	5.4	n.a.	[37, 72, 73]
mCardinale	603	651	79,000	18.0	14.2	229		n.a.	5.3	n.a.	[72–74]

^aDetermined as a product of extinction coefficient at excitation maximum (in mM⁻¹cm⁻¹) and fluorescence quantum yield.

^bDetermined as an effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence of co-transfected EGFP and overlap of FP spectra with excitation laser and emission filters.

^cCharacteristics of NIR FPs shown in original publications are in parentheses.

^dOriginally reported as a monomer, IFP2.0 was later found to be a dimer [22]

^eFarred GFP-like FP.

^fOriginally reported as monomers, mNeptune and mCardinal were later found to be dimers [73].

n.a., not available.