Fig. 3. Ribosomal protein S15a (RPS15a) expression after RPS15a silencing in each group as detected by western blot analysis. Western blot analysis of RPS15a protein expression in BGC823 cells transfected with different siRNAs and control cells demonstrated that the highest protein expression was present in the wild-type BGC cells and the lowest in the siRPS15a-2-transfected cells. ck, normal BGC823 cells; siNC, BGC823 cells transfected with the negative control sequence; siRPS15a-1 and siRPS15a-2, BGC823 cells transfected with siRPS15a-1- and siRPS15a-2-containing siRNA, respectively.