Fig. 8. Cell transfection efficiency and the effect of knocking down ribosomal protein S15a (RPS15a) on tumor growth in nude mice. (A) The fluorescence analysis shows that tumor cells were successfully transfected with the shRNA (magnification: ×100). (B) Quantitative PCR analysis of RPS15a mRNA expression in BGC823 cells transfected with shRNA and control cells (*p<0.01). (C) Western blot analysis of RPS15a protein expression in BGC823 cells transfected with shRNA and control cells demonstrated that the highest protein expression was present in the wild-type BGC cells. (D) Tumor growth curve (*p<0.01). (E) Tumor weight (*p<0.01). vector, BGC823 cells transfected with the empty plasmid; ck, normal BGC823 cells; shRPS15a, BGC823 cells transfected with the shRPS15a plasmid; pCDH, noncoding sequence-transfected BCG823 cells; rps15a-shRNA, RPS15a knock-down group.
