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. 2018 Nov 19;9(11):208. doi: 10.1038/s41424-018-0076-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Representative immunostaining of MMP-9 (left panel) and MMP-2 (right panel) in tissue biopsies from achalasia patients. Solid arrows depict MMP-9 expression (in brown). Dotted arrows show MMP-2 expression (in red). Original magnification was X320. Below the achalasia tissue samples, a normal biopsy from the esophagus of a transplant donor is included. The immunoreactivity for MMP-9 was with the monoclonal antibody REGA-2D9, whereas the immunoreactivity for MMP-2 was with a polyclonal antibody