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. 2018 Nov 12;8:399. doi: 10.3389/fcimb.2018.00399

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Copyright © 2018 Howe, Kordus, Cole, Bauman, Aldrich, Baughn and Minato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Methionine can affect but not bypass essentiality of upstream folate biosynthetic pathways in M. tuberculosis. (A) New working model of methionine-mediated PAS antagonism. (B) M. tuberculosis ΔpabB was grown to an OD₆₀₀ of ~0.5, washed three times with PABA-free 7H9 medium to remove residual PABA and resuspended in PABA-free 7H9 medium to a starting OD₆₀₀ of 0.01. Cultures were then supplemented with the indicated metabolites and incubated for 14 days with OD₆₀₀ readings taken at the given time points.