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. 2018 Nov 12;9:1588. doi: 10.3389/fphys.2018.01588

FIGURE 2.

FIGURE 2

(A) In vivo representation of developing kidney at embryonic day 15.5. SIX2 staining (pink) strongly localizes to the nephron progenitors in the cap mesenchyme (CM), while being less intensive in pretubular aggregate (PTA) and almost non-existent in renal vesicle (RV). Calbindin staining (green) highlights ureteric bud (UB) epithelium. Scale bar is 50 μm. (B) Schematic depiction of nephrogenesis. The UB is segmented into molecularly-distinct tip (T, olive green) and stalk (UB, yellow) regions. UB tips are surrounded by CM (dark blue), including the nephron progenitors and the surrounding stroma (S, brown). A subset of the nephron progenitors in CM are induced (iCM, turquoise) to differentiate. The first differentiating nephron precursor structure is a pre-tubular aggregate (PTA, gray), which through epithelialization, turns into an S-shaped body (SSB, green) that will eventually become the functional nephron. Vascular endothelial progenitor cells (VPCs, pink) enter the cleft of the S-shaped body to begin formation of the glomerular capillary bed.