FIGURE 1.

Phenotypic analysis of T cell subsets and activation markers in naive B6 WT and SIRT3^−/− mice. (A–H) Splenocytes were isolated from B6 WT or SIRT3^−/− mice (n = 6 mice per group) and analyzed for absolute total splenocyte numbers (A); the percentages and absolute numbers of CD4⁺ and CD8⁺ T cells (B); naive (CD44⁻CD62L⁺), effector memory (EM) T cell (EM: CD44⁺CD62⁻), and central memory (CM) T cell (CM: CD44⁺CD62L⁺) subsets (C); T_reg cells (CD4⁺Foxp3⁺) (D); memory precursor effector cells (CD8⁺KLRG^lowCD127^high) (E); terminal differentiated SLECs (CD8⁺KLRG^hiCD127^low) (F); and activation marker expression (CD25 and CD69) in CD4⁺ or CD8⁺ T cells (G and H). Error bars show the mean ± SEM from three independent experiments. *p < 0.05.