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. 2018 Nov 12;9:2494. doi: 10.3389/fimmu.2018.02494

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Copyright © 2018 Holder, Lajoie and Grant.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD16-dependent degranulation and IFN-γ production by Tim-3^pos NK cells in HIV infection. The percentage of cells expressing CD107a (left panels) or IFN-γ (right panels) within either the total or Tim-3^pos NK cell populations after CD16 stimulation was measured based on the gating strategy shown in Figure 5 and contrasted between (A) HCMV^neg (n = 9) and (B) HCMV^pos (n = 15–19) and between the (C) HCMV^neg, NKG2C^lo, and NKG2C^hi HIV-infected study groups. Error bars in (A) represent mean ± SD and comparison between conditions carried out with Student's paired t-test. Bars in (B) represent mean ± SD and Student's paired t-test performed (left panel) or median with IQR with significance calculated by Wilcoxon signed-rank test (right panel). Error bars in (C) represent mean ± SD where data was normally distributed and median with IQR in all other cases.