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. 2018 Nov 12;9:1630. doi: 10.3389/fpls.2018.01630

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Copyright © 2018 Etienne, Breton, Breitler, Bertrand, Déchamp, Awada, Marraccini, Léran, Alpizar, Campa, Courtel, Georget and Ducos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the Coffea arabica mass propagation process (24 months) through somatic embryogenesis (SE). The process is based on the use of liquid nutritive media for the embryogenic tissue multiplication and embryo regeneration phase and for the pre-germinated embryo and plantlet bulk production in temporary bioreactors (1L-RITA, 5L-MATIS and 3L-Twin-flasks). Direct sowing of germinated embryos and plantlets onto horticultural substrate avoids the use of gel media and requires less manpower in in vitro conditions.