Figure 2.

The expression of phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1) in HEI-OC1 cells was influenced by GM stimulus. (A) The expression of PINK1 in response to GM and GM plus NAC co-treatment were measured by use of Western blotting. (B) Analysis of gray-degree showed that the expression of PINK1 decreased immediately after GM treatment for 1 h, then increased step by step reaching the peak at 12 h followed by a decline at 24 h. The co-treatment of NAC could inhibit the changes of PINK1 expression induced by GM, as the changes of NAC co-treated groups showed no statistic difference in contrast to control group. NAC could effectively inhibit the decrease of PINK1 expression, especially at early period (1 h), ^#p < 0.05 vs. GM group at 1 h, *p < 0.05, **p < 0.01.