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. 2018 Nov 16;12(2):282–291. doi: 10.1016/j.tranon.2018.10.013

Figure 1.

Figure 1

miR-224 is significantly upregulated in sporadic and IBD-related colorectal carcinogenesis. (A) A significant miR-224 upregulation was observed by qRT-PCR in LG-IEN (P = .033), in HG-IEN (P < .001), and in early-stage CRCs (P < .001) in comparison to normal colon mucosa. Also, HG-IEN lesions and CRCs showed a significantly higher miR-224 expression in comparison to LG-IEN adenomas (both P < .001). (B) Representative ISH analysis pictures showing a significant miR-224 overexpression in HG lesions in comparison to LG adenomas (ORIGINAL magnifications 20×, scale bars 100 μm) and the relative expression as assessed by image software analysis. (C-D) miR-224 expression (qRT-PCR) was also investigated in a large series of IBD-related IEN (n = 39) and CRC (n = 36) lesions. In both ulcerative colitis (UC; C) and Crohn's disease (CD; D), miR-224 levels were significantly upregulated between inactive IBD disease or IEN and full-blown CRC (UC, P < .001 and P = .006, respectively; CD, P < .001 and P = .010, respectively). Although not significant, miR-224 expression was higher in IEN samples in comparison to inactive disease.