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. 2018 Aug 14;27(23):4036–4050. doi: 10.1093/hmg/ddy290

Figure 2.

Figure 2

CMT-associated AARS mutations affect cellular growth in yeast. (A) Yeast lacking endogenous ALA1 (the yeast ortholog of AARS) were transformed with vectors containing wild-type AARS or mutant AARS or a vector without AARS insert (‘empty’). Transformation was performed twice (transformation A,B). Resulting cultures were plated undiluted or diluted (1:10 or 1:100) on media containing 5-FOA and grown at 30°C. (B) Cultures resulting from the same transformations as in A were plated undiluted or diluted on media containing 5-FOA and grown at 37°C. (C) Conservation of the affected amino acid residues. The position of each variant is shown along with flanking AARS amino acid residues from multiple, evolutionarily diverse species. The position of the affected residue is shown in red for each species.