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Whole mount immunohistochemical staining demonstrated aberrant patterns of synaptotagmin expression. Expression of synaptotagmin (blue staining) as detected by antibody znp-1 in wild-type zebrafish at 3 dpf (A) and in zebrafish injected at 1-cell stage with control MO (B,C) or with splice-MO (D–G) at 3 dpf. The immunostaining shows a less defined and increasingly irregular pattern as morphology grows worse.
