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Injection of all three mutant mRNAs give aberrant morphologies after injection in embryos of zebrafish reporter lines while equal or larger amounts of wild-type mRNAs do not. Pictures were taken at 2 dpf after injection of 500 pg of mutant (A–C) mRNA or wt mRNA (E) in 1-cell stage embryos of reporter strains or of non-injected controls (D). Mutant injected mRNAs: c.1009G > A, p.Glu337Lys (A), c.1880C > T, p.Ser627Leu (B), c.976C > T, p.Arg326Trp (C).
