Fig. 1.

Design of the HSE-RFP reporter construct. (A) The reporter transgenic mouse harboring the Heat Shock-response Element (HSE) that contains the mouse Hsp70 promoter followed by RFP (DsRed2) coding sequence. When HSF1 is activated, it binds the HSE to transcribe RFP reporter. (B) Full sequence of HSE. Green letters indicate HSF binding domains. The details of reporter evaluation have been described previously (Torii et al., 2017). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).