Fig. 2.

HSE-RFP reporter induction in the spinal cord and cerebral cortex following spinal cord injury. (A–D) HSE-RFP reporter expression was detected by immunohistochemistry with 3,3'-Diaminobenzidine (black) in coronal (A and C, at the T9 level) and sagittal (B and D, lateral to the lesion) sections around the lesion site 4 days after spinal cord injury (C and D) and at the same regions in the sham control (A and B). HSE-RFP reporter expression was observed in cells surrounding the lesion site (C and D). Arrows in C and D indicates the regions with the reporter expression. Note that the center of section in C is damaged due to the lesion. The arrowhead in D indicates the level of the lesion. (E) Sagittal view of the brain with immunofluorescence labeling for Fluoro-Gold (FG, green) and RFP (red) 4 days after spinal cord injury. Strong reporter expression is observed in the FG⁺ backfilled corticospinal projection neurons in layer V (bracket) as well as cells in other layers in the M1 cortex. The inset shows a higher magnification view (from another sample) of FG⁺/RFP⁺ neurons in layer V and RFP⁺ neurons above/below layer V. (F) No reporter expression was observed in the M1 in sham brain labeled for RFP (red) and nuclei (DAPI stain, blue). M1: primary motor cortex, M2: secondary motor cortex, MPtA: medial parietal association cortex. Bars = 100 (A–D) and 200 (E and F) μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).