Table 2.
Secreted Frizzled Related Protein 1 (SFRP1) promoter methylation status, expression level of SFRP1, beta-catenin, lymphoid-enhancer factor 1 (LEF1), and T-cell factor 1 (TCF1) proteins in astrocytoma samples
| World Health Organization grade* |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| A II (diffuse astrocytoma) |
A III (anaplastic astrocytoma) |
G IV (glioblastoma) |
|||||||
| U | M/U | M/U | U | M/U | M/U | U | M/U | M/U | |
|
Methylation status of SFRP1 promoter |
6/6 |
5/6 |
1/6 |
6/13 |
3/13 |
3/13
1/13‡ |
|||
|
Expression status† |
1 |
2 |
3 |
1 |
2 |
3 |
1 |
2 |
3 |
|
SFRP1 |
3/6 |
3/6 |
2/6 |
2/6 |
2/6 |
6/12 |
4/12 |
2/12 |
|
|
Beta-catenin |
2/6 |
2/6 |
2/6 |
4/6 |
2/6 |
8/14 |
3/14 |
3/14 |
|
|
LEF1 |
1/3 |
2/3 |
2/3 |
1/3 |
1/11 |
2/11 |
8/14 |
||
| TCF1 | 2/3 | 1/3 | 2/2 | 1/3 | 4/11 | 2/11 | 5/11 | ||
*M – methylated; U – unmethylated; bold – pronouncedly present; regular – present.
†1 – weak or lack of expression; 2 – moderate expression; 3 – strong expression.
‡Sample with same band intensities.