Figure 7.

PeIA residue Pro¹³ is an important determinant of PeIA potency for α6/α3β4 nAChRs. X. laevis oocytes expressing human or rat α6/α3β4 nAChRs were subjected to TEVC electrophysiology as described under “Experimental procedures,” and the IC₅₀ values were determined for inhibition of ACh-evoked currents by analogs of PeIA. A, concentration–response curves for inhibition of human α6/α3β4 nAChRs by analogs of PeIA. B, concentration–response curves for inhibition of rat α6/α3β4 nAChRs by analogs of PeIA. Values are provided in Table 5. Error bars, S.D. from at least four individual oocytes for each IC₅₀ determination. Data for inhibition of human α6/α3β4 (dashed red) and rat α6/α3β4 nAChRs (dashed green) by PeIA were presented previously and shown for ease of visual comparison.