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UDP-5S-GlcNAc modification of HCF-1PRO-repeat threonine-rich region–containing peptides. CK2 (A), HCF-1PRO-repeat 2 E10S (B), Ch13 (C), and Ch13 T(17–22)A peptides (D) were incubated with WT OGT and either UDP-GlcNAc (blue) or UDP-5S-GlcNAc (red). Peptide glycosylation was detected using LC-MS. The highest peak in each pair of samples was set at 100%. Single representative examples of three independent experiments are shown.
