Figure 2.

Liver-specific GCN5L1 Tg mice display elevated mitochondrial protein acetylation. A, sequence and putative start sites of GCN5L1 isoforms. Underlined sequence denotes GCN5L1 antibody epitope. B, GCN5L1 is present in two isoforms in the liver, lung, and spleen. C and D, only the 15-kDa protein isoform is present in mitochondria (P1 fraction). E and F, mice overexpressing mitochondrial GCN5L1 under the albumin promoter display increased mitochondrial protein acetylation in the liver. G, proteins that share elevated hepatic acetylation levels at a specific identified lysine residue in both GCN5L1 Tg–overexpressing and SIRT3 knockout mice. *, fatty acid oxidation–related protein. WCL, whole-cell lysate.