Figure 3.

Liver HADHA acetylation is elevated following short-term HFD exposure. A, HADHA co-immunoprecipitates with GCN5L1 in mouse livers. The arrow denotes a nonspecific band at a higher molecular weight in the control IgG fraction. B and C, HADHA displays elevated levels of lysine acetylation in the livers of mice fed a HFD for 1 week. D–F, knockdown of GCN5L1 expression in HepG2 cells significantly decreased HADHA acetylation levels. shRNA line 2 was used for subsequent experiments. G–I, loss of GCN5L1 expression increases fatty acid oxidation enzyme activity in HepG2 cell protein lysates. n = 4; *, p < 0.05. WB, Western blotting; IP, immunoprecipitation; Ac-K, acetyl-lysine.