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. 2018 Sep 27;293(46):17906–17916. doi: 10.1074/jbc.RA118.004510

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© 2018 Ndeh et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Evidence of BtCDH endo-lyase activity. A, TLC showing progressive degradation of hyaluronanic acid (HA_L, 10–20 kDa) by BtCDH lyase over time. For the reaction, 32 mg/ml of HA_L was digested with 2 μm of enzyme. The reaction was stopped at the various time points indicated, and 64 μg of each sample was analyzed by TLC. B, mass spectrometry data of purified HA_L oligosaccharide species. Panel 1, spectrum for Δ^4,5UA-disaccharide (Δ^4,5UA-Di or band A). Panel 2, spectrum for Δ^4,5UA-octasaccharide (Δ^4,5UA-Octa or band B). Panel 3, spectrum for Δ^4,5UA-tetrasaccharide (Δ^4,5UA-Tetra). Panel 4, spectrum for Δ^4,5UA-hexasaccharide (Δ^4,5UA-Hexa). C, structural representation of various species detected in B.