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. 2018 Sep 27;293(46):17906–17916. doi: 10.1074/jbc.RA118.004510

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© 2018 Ndeh et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Activity of BtCDH against various chondroitin sulfate oligosaccharides. Substrates (1 mg/ml each) were treated with 0.5 μm of BtCDH (shown as DPx +, with x being the number of constituent monosaccharides) overnight or diluted in equivalent volume of buffer as control (DPx). HPAEC chromatograms show major shifts in the signals of sulfated DP10 and DP12 oligosaccharides following treatment, accompanied by the appearance of a strong signals for the Δ^4,5UA-GalNAc disaccharide in each case.