Figure 5.

Effect of temperature and pH and metals on BtCDH lyase activity. A, effect of temperature. Enzymatic activity was measured using 40 μm of CSA as substrate in 20 mm NaH₂PO₄ at pH 7.0 over different temperatures. B, effect of pH. BtCDH activity was measured at different pH levels using 20 mm of various buffers at 37 °C. C, effect of various metals. The reactions were carried out in 20 mm NaH₂PO₄ buffer containing 5 mm EDTA, Mg²⁺, Ca²⁺, Mn²⁺, and Co²⁺ and 1 mm Zn²⁺ and Ni²⁺ in separate experiments.