Table 3.

Thermal stabilities of the wild type and mutant CTX-M enzymes determined by CD

Variant	Tm	ΔTm	ΔΔG	ΔH
	°C	°C	kcal/mol	kcal/mol
CTX-M-14	52.8 ± 0.01			221 ± 2
N106S	58.0 ± 0.04	+5.2	3.20 ± 0.13	212 ± 9
D240G	49.6 ± 0.81	−3.2	−1.59 ± 0.37	152 ± 7
N106S/D240G	57.4 ± 0.23	+4.6	2.49 ± 0.04	187 ± 8