Figure 1.

Low biological and technical variability between pooled samples processed together. (A) Schematic of processing of dispersed cells from six pituitary glands for single-cell capture and RNA sequencing. (B) tSNE plots for each pool show highly similar cell signatures, and there are no clusters that are present in only one pool. (C) Merged tSNE plots for the two pools show high overlap between the clusters from each pool. (D) Volcano plot of differentially expressed genes between the two pools. No genes are significantly differentially expressed.