Figure 3.

Measurements of proteins involved in Gr1⁺CD11b⁺ cell expansion in sepsis. Gr1⁺CD11b⁺ cells were isolated from the bone marrows of sham and septic mice (n = 6 mice per group). (a) p21 is expressed in sepsis Gr1⁺CD11b⁺ cells from Nfia knockout mice. Gr1⁺CD11b⁺ cell extracts were prepared, and levels of NFI-A and p21 proteins were determined by Western blot. (b) Reversal of CDK4, PTEN and NF-κBp65 protein expression patterns in sepsis Gr1⁺CD11b⁺ cells from NFI-A knockout mice. Levels of CDK4, PTEN and NF-κBp65 proteins in Gr1⁺CD11b⁺ cells were determined by Western blot. The results represent one of three experiments. cKO: conditional knockout.