Table 3.
Production levels of 11-oxo-13R-manoyl oxide (2) and 11-hydroxy-13R-manoyl oxide (3) by CYP76AH15 variants with SRS mutations using microtiter plates for yeast growth
| Variant | SRS site(s) | 2a | 3a |
|---|---|---|---|
| Native CYP76AH15 | – | 1 | 1 |
| A99I | 1 | 5.6 | 3.0 |
| S235G Y236F | 3 | 5.1 | 7.3 |
| L366F | 5 | 2.9 | 4.2 |
| L366E | 5 | 3.7 | 6.2 |
| A99I S235G Y236F | 1 + 3 | 6.5 | 14.2 |
| A99I L366F | 1 + 5 | 6.2 | 4.9 |
| S235G Y236F L366E | 3 + 5 | 3.1 | 16.5 |
| A99I S235G Y236F L366F | 1 + 3+5 | 3.2 | 31.4 |
aFold change of products 2 and 3, respectively, compared to native CYP76AH15