Table 4.
Production titers of 13R-manoyl oxide (1), 11-oxo-13R-manoyl oxide (2) and 11-hydroxy-13R-manoyl oxide (3) by CYP76AH15 variants with SRS mutations using shake-flasks for yeast growth
| Name | Total mg/L | Oxygenated (%) | 1 mg/L | 2 mg/L | 3 mg/L | Content of 2 (%) |
|---|---|---|---|---|---|---|
| MO (−) | 43.8 | 0 | 43.8 | 0.0 | 0.0 | 0 |
| WT CYP76AH15 | 43.8 | 64 | 15.9 | 23.4 | 0.2 | 53 |
| A99I | 87.7 | 99 | 0.9 | 86.4 | 0.4 | 99 |
| S235G Y236F | 73.5 | 94 | 4.1 | 68.6 | 0.8 | 93 |
| L366F | 68.2 | 96 | 3.1 | 64.7 | 0.5 | 95 |
| L366E | 69.2 | 90 | 7.0 | 61.2 | 1.0 | 88 |
| A99I S235G Y236F | 83.4 | 97 | 2.2 | 79.8 | 1.4 | 96 |
| A99I L366F | 75.8 | 96 | 2.8 | 72.6 | 0.4 | 96 |
| S235G Y236F L366E | 47.1 | 80 | 9.6 | 34.2 | 3.3 | 73 |
| A99I S235G Y236F L366F | 59.6 | 83 | 10.2 | 44.2 | 5.1 | 74 |